The role of PE/PPE proteins in tuberculosis

Tuberculosis remains one of the world’s biggest killers due to an infectious disease. There remains an urgent need for the development of effective vaccines and novel drug targets against the disease. The PE/PPE family of proteins from Mycobacterium tuberculosis make up about 10% of the pathogen’s proteome. These proteins play pivotal roles in the interaction between the pathogen and its host, influencing virulence, immune evasion, and drug resistance (D’Souza et al., 2023). The aim of this project is to characterise several members of this family whose functions are unknown using in vitro and in vivo approaches.

Training Opportunities:

• Gain hands-on experience in cutting-edge molecular microbiology techniques, including gene manipulation, protein expression and purification, CRISPRi, dual RNA-Seq, Flow Cytometry and FACS and Fluorescence Microscopy.
• Utilise advanced Bioinformatics tools for sequence analysis and structural modelling to elucidate protein function and interactions.
• Collaborate with interdisciplinary teams, fostering skills in scientific communication, leadership, and project management.
• Access state-of-the-art facilities and resources to support your research goals.
• Present your findings at national and international conferences, enhancing your visibility within the scientific community.

Preferred Candidate Profile:

We are seeking a highly motivated and enthusiastic individual with a strong background in Microbiology, Genomics, Biochemistry, Immunology, Molecular Biology, or a related field.

The ideal candidate should possess:

• A Bachelors or Masters degree (or equivalent) in a relevant discipline.
• Proficiency in laboratory techniques, with prior experience in bacterial genetics and protein biochemistry preferred.
• Excellent analytical and problem-solving skills, coupled with a passion for scientific inquiry.
• Effective communication abilities and the ability to work collaboratively in a team environment.
• A keen interest in infectious diseases and a drive to make a meaningful impact on global health.

References:

D'Souza C, Kishore U, Tsolaki AG. The PE-PPE Family of Mycobacterium tuberculosis: Proteins in Disguise.
Immunobiology. 2023 Mar;
228(2):152321.
doi: 10.1016/j.imbio.2022.152321.
Epub 2022 Dec 30.

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